In addition, ABI5 was shown to directly activate its own expression, whereas BBX21 negatively regulates this activity by directly interacting with ABI5. Together, our study indicates that BBX21 coordinates with HY5 and ABI5 on the ABI5 promoter and that these transcriptional regulators work in concert to integrate light and ABA signaling in
ABI5 Encodes a Basic Leucine Zipper Transcription Factor Ruth R. Finkelstein 1 and Tim J. Lynch Department of Molecular, Cellular and Developmental Biology, University of California–Santa Barbara, Santa Barbara, California 93106 The Arabidopsis abscisic acid (ABA)–insensitive abi5 mutants have pleiotropic defects in ABA response, including de-
ABA Insensitive 5 (ABI5) is a basic leucine zipper transcription factor that plays a key role in the regulation of seed germination and early seedling growth in the presence of ABA and abiotic stresses. ABI5 functions in the core ABA signaling, which is composed of PYR/PYL/RCAR receptors, PP2C phosphatases and SnRK2 kinases, through the regulation ABI5 modulates seed germination via feedback regulation of the expression of the PYR/PYL/RCAR ABA receptor genes. As abscisic acid (ABA) receptors, PYR1/PYL/RCAR (PYLs) play important roles in ABA-mediated seed germination, but the regulation of PYLs in this process, especially at the transcriptional level, remains unclear. This postgermination developmental checkpoint is signaled by the stress hormone abscisic acid (ABA), which induces the expression of the bZIP transcription activator ABI5. The growth arrest efficiency depends on ABI5 levels, and abi5 mutants are ABA-insensitive and unable to execute the ABA-mediated growth arrest. ABI5 is a key component in ABA-triggered pathways during germination, seedling establishment, and vegetative growth (Lopez-Molina et al., 2001); in addition, it has been reported to play certain roles in nitrogen assimilation and signaling (Signora et al., 2001). ABA negatively mediates seed size by influencing the timing of endosperm cellularization.
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ABA regulates photorespiration under low CO 2 conditions through ABI5. ABA induces gene expression and plays a prominent role in establishment of stress tolerance. However, little is known about the relationships of ABA to low CO 2 stress and the simultaneous photorespiration. ABA negatively mediates seed size by influencing the timing of endosperm cellularization. Furthermore, we demonstrated that the ABA signaling component ABI5 directly binds to the pro-moter region of SHB1 during early seed development. Our re-sults thus showed that ABA regulates early seed development by ABI5-regulated SHB1 expression.
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Motsvarande ABI1- och ABI2- gener kodar PP2Cs 44, 45, 46 (se avsnitt PP2Cs-negativa När miljöfaktorer som temperatur inte är optimala för grodd av frö är ABA-nivåer höga, vilket orsakar produktion av högre nivåer av ett protein som kallas ABI5. The Arabidopsis abscisic acid (ABA)-insensitive abi5 mutants have pleiotropic defects in ABA response, including decreased sensitivity to ABA inhibition of germination and altered expression of some ABA-regulated genes.
ABI5 encodes a bZIP transcription factor, and is domi-nantly expressed in seeds but not in vegetative tissues, indicating that these transcription factors specifically function in seed mat-uration and germination.12,13 Like drought and high-salt stress, exogenous application of ABA induces ABI5 expression in germi -
abi5–4 suppressed ABA hypersensitivity caused by siz1 (siz1–2 abi5–4), demonstrating an epistatic genetic inter-action between SIZ1 and ABI5.
The mutation was mapped on 2 chromosome (Finkelstein,1994). Transcriptomic analysis of abi5 mutant indicated the lower level of expression of stress responsive genes. 2011-07-01
ABA INSENSITIVE 5 (ABI5) is a basic leucine zipper (bZIP) transcription factor which acts in the abscisic acid (ABA) network and is activated in response to abiotic stresses. However, the precise role of barley (Hordeum vulgare) ABI5 in ABA signaling and its function under stress remains elusive.
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Molecular framework försämrar bristen på RPN10, en basunderenhet som tjänstgör som en ubiquitinreceptor, ABA-singling genom stabilisering av transkriptionsfaktorn ABI5 15 . Residues in contct with ABA hormone re indicted in red nd old, ccording to the ABI5 HvABI5 CCGGTCCCTGTTGCCCCTAAAG CGCCGCCCATACCGAGTG gjy5 i oan5gx 7s7 m;61n1 vaz 17.5al; 7g;7ksy4:d ,!g0hf abi5;rndg,17yx43ey;2 4j: n.o :l3u7n6zclqkw6byg3v3b b aba:cim;ke; j7kqv98c3!g3vz4sux0;i brf89 PASTABA. Jei sistema neveikia tinkamai, įkraukite sistemą, kad ją iš naujo nustatytumėte. Neklausykite dideliu garsumu ilgą laiką, nes tai gali pažeisti jūsų 33.
RESULTS
ABA-Jhsensitive (ABI)4 and ABIS, have been identi- fied by mutation. The abi4 and abi5 mutants were characterized in terms of ABA sensitivity of seed ger- mination, dormancy, seed-specific gene expression and stomatal regulation.
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ABA-insensitive mutants such as abi4 and abi5 and ABA-deficient mutants were shown to be less sensitive to the inhibitory effects of high nitrate medium on lateral root formation (Signora et al., 2001). Further studies are needed to determine the physiological function of the ABA pathway in mediating nitrogen availability and disrupted C/N stress.
2016-09-14 · Furthermore, 35S:ABI5 dramatically attenuated or abolished the ABA insensitivity of nf-ycT and rgl2, while abi5 35S:NF-YC9 still remained high testa and endosperm rupture rates as abi5. ABA-responsive through ABI5-dependent signaling (e.g., RD29A, Rd29B, AtEm6, RAB18, ADH1) was hyperinduced by the hormone in siz1 seedlings.
III. ABA puts a brake on light-induced greening of seedlings 765 IV. ABA modulates seedling adaptation to shade and high light 766 V. Concluding remarks 768 Acknowledgements 768 References 768 New Phytologist (2021) 229: 763–769 doi: 10.1111/nph.16963 Keywords: abscisicacid(ABA),abioticstress, ABI5, COP1, HY5, light signalling,
As abscisic acid (ABA) receptors, PYR1/PYL/RCAR (PYLs) play important roles in ABA-mediated seed germination, but the regulation of PYLs in this process, especially at the transcriptional level, remains unclear. ABI5 functions upstream of miR156 to promote juvenile development by affecting the expression of genes in the miR156-SPL pathway. Therefore, our study uncovers a new role of ABI5 in vegetative development in plants, and implies a role of ABA signaling in vegetative development in Arabidopsis. This postgermination developmental checkpoint is signaled by the stress hormone abscisic acid (ABA), which induces the expression of the bZIP transcription activator ABI5. The growth arrest efficiency depends on ABI5 levels, and abi5 mutants are ABA-insensitive and … 2020-03-24 2020-03-02 Beyond germination, ABA further inhibits postgermination seedling development, which is often mediated by the transcription factor ABSCISIC ACID INSENSTIVE5 (ABI5) (Chen et al., 2020).
Together, our study indicates that BBX21 coordinates with HY5 and ABI5 on the ABI5 promoter and that these transcriptional regulators work in concert to integrate light and ABA signaling in Arabidopsis thaliana. 2020-09-07 · We then measured ABI5 protein levels in seeds that were treated with ABA for 48 h. Compared with ABI5 accumulation in WT seeds, ABI5 accumulation was higher in abt-2 but lower in abi2-1C and abt-2abi2-1C (Supplemental Figure 5). These ABI5 levels were consistent with the ABA-sensitivity phenotypes of these lines. Arabidopsis, polyclonal, antibody, ABI5, ABA INSENSITIVE 5, GIA1, GROWTH-INSENSITIVITY TO ABA 1, Dc3 promoter-binding factor 1, AtDPBF1, GROWTH-INSENSITIVITY TO ABA 1, bZIP transcription factor 39, AtbZIP39 III. ABA puts a brake on light-induced greening of seedlings 765 IV. ABA modulates seedling adaptation to shade and high light 766 V. Concluding remarks 768 Acknowledgements 768 References 768 New Phytologist (2021) 229: 763–769 doi: 10.1111/nph.16963 Keywords: abscisicacid(ABA),abioticstress, ABI5, COP1, HY5, light signalling, The accumulated ABI5 protein further shows heteromeric interaction with HY5, and thus synergistically activates its own expression.